超便携式调制叶绿素荧光仪——MINI-PAM-II

主要功能

• 可测荧光诱导曲线并进行淬灭分析

• 可测光响应曲线和快速光曲线（RLC）

• 全部采用 LED 光源，全新触摸屏设计

• 新增湿度测量功能，新增远红光源

• 仪器更加便携，适合野外测量

• 可通过微光纤与光合仪（如 GFS-3000）联用

• 采用5号电池供电，备用电池易获得，适合长期野外使用

测量参数

Fo, Fm, F, Fm', Fo’, Fv/Fm, Y(II) 即 ΔF/Fm', qP, qL, qN, NPQ, Y(NPQ), Y(NO), rETR, PAR、叶温和相对湿度等。

应用领域

研究光合作用机理、各种环境因子（光、温、营养等）对植物生理生态的影响、植物抗逆性（干旱、冷、热、UV、病毒、污染等）、植物的长期生态学变化等。

在植物生理学、植物生态学、植物病理学、农学、林学、园艺学、水生生物学、环境科学等领域有着广泛应用。

主要技术参数

• 测量光：蓝光 LED（470 nm）或红光 LED（655nm），光强 0.05 µmol m-2 s-1。

• 光化光：蓝光 LED（470 nm）或红光 LED（655nm），最大连续光强 3000 μmol m-2 s-1。

• 饱和脉冲：蓝光 LED（470 nm）或红光 LED（655nm），最大闪光强度 6000 μmol m-2 s-1。

• 远红光：发射峰值 740 nm。

• 信号检测：PIN-光电二极管，带长通和短通滤光片，带选择性锁相放大器。

• 数据存储：8M 存储卡，可存储 27000 组数据。  

• 叶夹：2035-B 叶夹包含 MINI-PAM/ F 光纤放置口和样品夹。叶夹上下两部分打开后可以夹住叶片。夹子上面部分提供了一个直径为 1 厘米圆形测量面积。光纤尖顶与测量区域之间的标准距离为 8 mm。光纤与测量平面呈 60° 角。叶片温度传感器安装在测量区域下方。湿度传感器安装在距离测量区域 3cm 的位置。内置芯片保存传感器的校准数据。饱和脉冲可以通过遥控触发按钮被释放。另外提供一个附加光传感器输入接口。PAR 测量范围 0-7000 µmol m-2 s-1，叶片温度测量范围 -20 至 +60℃，湿度测量范围 0-100％RH。

• 供电：6 节 AA（5 号 1.2 V/2 Ah）可充电电池，充电一次可供 1000 次饱和脉冲闪光。

选购指南

一、高等植物叶片测量基本款

系统组成：主机，光纤，光适应叶夹，暗适应叶夹，软件等

注意：高等植物叶片红光版和蓝光版（推荐）主机可选其一

高等植物叶片测量基本款

二、悬浮样品测量基本款

系统组成：主机，光纤，悬浮样品室，磁力搅拌器，光纤测氧仪（选配），软件等

注意：藻类测量时，建议选择红光版主机

悬浮样品测量基本款

初始界面	基本测量结果显示界面	详细参数显示界面
实时荧光显示界面	慢速诱导动力学曲线界面	快速光曲线显示界面

三、其他可选附件

• 外置 LED 光源：2054-L。

• 可以连接到 2035-B 叶夹上提供外置光化光，红绿蓝白 4 色，最大激发波长分别为 630 nm 红光, 520 nm 绿光, 452 nm 蓝光和波长范围 450nm-680nm 的白光。每种波长的光标准最大光强为 1500µmol m-2 s-1 连接外部供电装置情况下，整体最大光强可达 6000µmol m-2 s-1 颜色组合可以自由选择。

• 90 度角光纤适配器：2030-B90 。

• 安装在 2030-B 或 2060-B 上，使光纤与样品成 90 度角。

• 微型光量子/温度传感器：2065-M。

• 测量 PAR 和温度，可连接 MINI-PAM 后独立使用，多与 2060-B 结合使用。

• 大样品表面光纤固定支架：2060-A。

• 将光纤固定在较大样品的表面（非叶片），与 2065-M 结合使用。

• 拟南芥叶夹：2060-B。

• 60 度角光适应叶夹，与独立微型光量子/温度传感器 2060-M 连用进行测量，特别适于测量拟南芥类小叶片。需配置 2060-M。

• 微光纤：MINI-PAM/F1。

• 直径 2 mm，长 1.5 m，用于测量小样品。包括连接到 2035-B 的适配器。

应用示例

产地：德国WALZ

参考文献

数据来源：光合作用文献 Endnote 数据库，更新至 2021 年 1 月，文献数量超过 10000 篇

原始数据来源：Google Scholar

注：MINI-PAM-II 为 MINI-PAM 新升级产品，更多文献可参考 MINI-PAM 文献目录

Gong, X., et al. (2021). "Intercropping combined with nitrogen input promotes proso millet (Panicum miliaceum L.) growth and resource use efficiency to increase grain yield on the Loess plateau of China." Agricultural Water Management 243: 106434.

Afzal, M. J., et al. (2020). "Combined application of Bacillus sp. MN-54 and phosphorus improved growth and reduced lead uptake by maize in the lead-contaminated soil." Environmental Science and Pollution Research.

Andrzejczak, O. A., et al. (2020). "The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming. ." Int. J. Mol. Sci(21): 1546.

Casadesús, A., et al. (2020). "Differential accumulation of tocochromanols in photosynthetic and non-photosynthetic tissues of strawberry plants subjected to reiterated water deficit." Plant Physiology and Biochemistry 155: 868-876.

CHEN Li-li, W. H.-y., GONG Xiao-chen, ZENG Zhao-hai, XUE Xu-zhang, HU Yue-gao (2020). "Transcriptome analysis reveals effects of red and blue light-emitting diodes (LEDs) on the growth, chlorophyll fluorescence and endogenous plant hormone of potato (Solanum tuberosum L.) plantlet cultured in vitro." 2020: 0-.

Czarnes, S., et al. (2020). "Impact of soil water content on maize responses to the plant growth-promoting rhizobacterium Azospirillum lipoferum CRT1."  206(5): 505-516.

Hu, L., et al. (2020). "Microplastics generated under simulated fire scenarios: Characteristics, antimony leaching, and toxicity." Environmental Pollution: 115905.

Huang, D., et al. (2020). "Silencing MdGH3-2/12 in apple reduces cadmium resistance via the regulation of AM colonization." Chemosphere: 129407.

Kaiser, E., et al. (2020). "Photorespiration Enhances Acidification of the Thylakoid Lumen, Reduces the Plastoquinone Pool, and Contributes to the Oxidation of P700 at a Lower Partial Pressure of CO2 in Wheat Leaves." Plants (Basel) 9(3): 319.

Khan, H., et al. (2020). "Effective Control against Broadleaf Weed Species Provided by Biodegradable PBAT/PLA Mulch Film Embedded with the Herbicide 2-Methyl-4-Chlorophenoxyacetic Acid (MCPA)." ACS Sustainable Chemistry & Engineering 8(13): 5360-5370.

Kim, J.-H., et al. (2020). "Global warming offsets the ecophysiological stress of ocean acidification on temperate crustose coralline algae." Marine pollution bulletin 157: 111324.

Liu, C., et al. (2020). "Low-nitrogen tolerance comprehensive evaluation and physiological response to nitrogen stress in broomcorn millet (Panicum miliaceum L.) seedling." Plant Physiology and Biochemistry 151: 233-242.

Martins, J. P. R., et al. (2020). "Morphophysiological responses, bioaccumulation and tolerance of Alternanthera tenella Colla (Amaranthaceae) to excess copper under in vitro conditions." Plant Cell, Tissue and Organ Culture (PCTOC).

O'Brien, M. J., et al. (2020). "Foundation species promote local adaptation and fine-scale distribution of herbaceous plants."  n/a(n/a).

O’Connell, E. and J. Savage (2020). "Extended leaf phenology has limited benefits for invasive species growing at northern latitudes." Biological Invasions.

P.S, C., et al. (2020). "Cadmium biosorption and biomass production by two freshwater microalgae Scenedesmus acutus and Chlorella pyrenoidosa: An integrated approach." Chemosphere: 128755.

Pérez-Molina, J., et al. (2020). "Chlorophyll fluorescence and biomass partitioning within light and nitrogen deficiency: An example of the use of the R programming language for teaching." UNED Research Journal 12: 2629.

Rasool, G., et al. (2020). "Effect of Buried Straw Layer Coupled with Fertigation on Florescence and Yield Parameters of Chinese Cabbage Under Greenhouse Environment." Journal of soil science and plant nutrition.

Röhlen-Schmittgen, S., et al. (2020). "Boosting leaf contents of rutin and solanesol in bio-waste of Solanum lycopersicum." Plant Physiology and Biochemistry.

Rosa Guadalupe, P.-H., et al. (2020). "Physiological and microclimatic consequences of variation in agricultural management of maize." Botanical Sciences(0).

Setsungnern, A., et al. (2020). "A defect in BRI1-EMS-SUPPRESSOR 1 (bes1)-mediated brassinosteroid signaling increases photoinhibition and photo-oxidative stress during heat stress in Arabidopsis." Plant Science 296: 110470.

Sinclair, M. N., et al. (2020). "Seasonal facilitative and competitive trade-offs between shrub seedlings and coastal grasses." Ecosphere 11(1): e02995.

Song, H., et al. (2020). "Differences in relative air humidity affect responses to soil salinity in freshwater and salt marsh populations of the dominant grass species Phragmites australis." Hydrobiologia.

Wada, S., et al. (2020). "Photorespiration Enhances Acidification of the Thylakoid Lumen, Reduces the Plastoquinone Pool, and Contributes to the Oxidation of P700 at a Lower Partial Pressure of CO2 in Wheat Leaves." Plants(9): 319.

Wang, L., et al. (2020). "Chloride salinity in a chloride-sensitive plant: Focusing on photosynthesis, hormone synthesis and transduction in tobacco." Plant Physiology and Biochemistry.

Wongnoi, S., et al. (2020). "Physiology, Growth and Yield of Different Cassava Genotypes Planted in Upland with Dry Environment during High Storage Root Accumulation Stage." Agronomy(10): 576.

Yañez-Serrano, A. (2020). "Heat Waves Change Plant Carbon Allocation Among Primary and Secondary Metabolism Altering CO2 Assimilation, Respiration, and VOC Emissions." Frontiers in Plant Science 11.

Zhang, Q., et al. (2020). "Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses." BMC Plant Biology 20(1): 315.

Zhang, X., et al. (2020). "Photosynthetic Properties of Miscanthus condensatus at Volcanically Devastated Sites on Miyake-jima Island." Plants(9): 1212.

Zhong, C., et al. (2020). "Ultrastructural evolution, physiological traits, and carbon and nitrogen assimilation–related gene expression are compatible with the developmental transitions of parthenogenesis in Pyropia haitanensis (Bangiales, Rhodophyta)." Journal of Applied Phycology.

Zou, W., et al. (2020). "The Influence of Size and Phase on the Biodegradation, Excretion, and Phytotoxicity Persistence of Single-Layer Molybdenum Disulfide." Environmental Science & Technology.

Hatam, Z., et al. (2020). "Zinc and potassium fertilizer recommendation for cotton seedlings under salinity stress based on gas exchange and chlorophyll fluorescence responses." South African Journal of Botany 130: 155-164.

Son, K.-H., et al. (2020). "Growth characteristics and phytochemicals of canola (Brassica napus) grown under UV radiation and low root zone temperature in a controlled environment." Horticulture, Environment, and Biotechnology.

Wang, Q.-W., et al. (2020). "Testing trait plasticity over the range of spectral composition of sunlight in forb species differing in shade tolerance." Journal of Ecology n/a(n/a).