蜂巢矩阵叶绿素荧光成像系统

蜂巢矩阵叶绿素荧光成像系统

HEXAGON-IMAGING-PAM

叶绿素荧光成像系统的“六边形战士"

精度高，面积大，功能全，应用广，文献多，数据可视化！

HEXAGON-IMAGING-PAM是德国WALZ公司最新推出的大型蜂巢矩阵叶绿素荧光成像系统。它凭借高精度的脉冲振幅调制（PAM）技术，可以对20×24cm的区域进行成像。分辨率高达1.2 MP（1000 x 1200 px, 2x2 binning技术，实际是2000×2400），像素尺寸3.45 x 3.45 µm。

超高分辨率的基础是成像区域光场的均匀性，在设计过程中，光源阵列中LED的位置是经过精心布局的，以保证测量区域内无阴影，所有成像区域内的样品均匀照光，样品间的差异可以尽收眼底。大功率LED面板的冷却效果非常好，可以最大限度的延长LED的使用寿命。

增加远红光（FR）LED 面板，可用于测量所研究样品的Fo'值。

HEXAGON-IMAGING-PAM采用蜂巢矩阵式LED面板拼接技术，单个六边形蜂巢矩阵单元之间LED的不平横可以独立补偿，初衷是为实现样品区域的理想照明提供更优选择。

尽管成像区域很大，但是它依然足够灵活，可以测量各种类型的样品，如盆栽植物，穴盘中培养的植物，培养皿上的植物或多孔板中的藻类悬浮液。

滑动门设计，集成安全关闭功能，开门状态下，饱和脉冲的强度会被抑制以保护操作人员的眼睛。

主要功能

l 原位测量：活体植物叶绿素荧光成像，直观显示样品光合作用光能利用差异，可导出彩色图像。

l 成像功能：对Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、PS/50=ETR、Inh等参数进行成像分析。测定调节性能量耗散Y(NPQ)，反映植物光保护能力，测定非调节性能量耗散Y(NO)，反映植物光损伤程度。

l 程序测量功能：可自动程序测量荧光诱导曲线、快速光曲线和暗弛豫，也可手动测量；在测量过程中能自动分析所有荧光参数的变化趋势；可以预编程进行自定义实验流程，如模拟波动光。

l AOI功能：可在测量前或测量后任意选择感兴趣的区域（AOI），程序将自动对选择的AOI的数据进行变化趋势分析，并在报告文件中显示相关AOI的数据。所有报告文件中显示的数据都可导出到EXCEL文件中。

l 成像异质性分析功能：对任意参数任意时间的成像，可在图像上任意选取两点，软件自动对两点间的数据进行横向异质性分析，并可导出到EXCEL文件中。

l 成像数据范围分析功能：对任意参数任意时间的成像，可分析任意两个荧光数值之间有多少个像素点，多少面积（cm2）。

l 突变株筛选功能：可跟据成像结果快速筛选光合、产氢/油、抗逆（抗盐、抗旱、抗病等）等突变株。

l 微藻毒理研究功能：可同时测量4块96孔板，即384个微藻样品（对照和处理组）的光合活性，软件自动给出处理组样品相对于对照组的光合抑制百分比。

应用领域

l 光合作用研究：可以在*相同的条件下同时对大量样品进行成像

l 植物病理学：病斑部位（包括肉眼不可见时）成像以及病斑扩散的时空动力学

l 植物胁迫生理学：肉眼不可见生物/非生物胁迫损伤的早期检测

l 遗传育种：出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

l 突变株筛选：快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

l 微藻毒理学：不同毒物浓度多个重复的样品一次测完，软件自动计算抑制比率

l 其它多种扩展研究

成像参数

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), PS/50=ETR,Inh.等

产地：德国WALZ

参考文献

数据来源：光合作用文献Endnote数据库，原始数据来源：Google Scholar。

注：HEXAGON-IMAGING-PAM为最新产品，暂无文献发表，最新研究成果可参考M-IMAGING-PAM发表文章。

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